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Figure 2 | BMC Cancer

Figure 2

From: Transcriptional profiling of ErbB signalling in mammary luminal epithelial cells - interplay of ErbB and IGF1 signalling through IGFBP3 regulation

Figure 2

Hierarchical clustering of 775 differentially expressed genes. Ratios of normalized values were used to show relative gene expression in two ways: (i) the EGF or HRG ratio (T*/T0) representing relative gene expression at each timepoint in relation to the untreated control, measuring the response to each ligand in HB4a and C3.6 cells separately, and (ii) the ErbB2 ratio (C3.6/HB4a) representing relative gene expression in C3.6 vs. HB4a at each time point, identifying genes whose expression are affected by ErbB2. Ratios were log2 transformed and loaded into TIGR MeV software v2.2 (Institute for Genomic Research) and unsupervised, average-linkage hierarchical clustering performed. Clusters indicated by light green and pink bands (on right) show genes whose expressions were altered by ErbB2 overexpression, but were similarly up- or down-regulated by EGF and HRG; the light blue cluster shows genes down-regulated by ErbB2, but up-regulated by EGF; the dark green cluster shows genes that were down-regulated by both growth factors and the red cluster shows genes that were transiently-induced in all treatments except in HRG-treated HB4a cells.

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