Figure 3
ADAMTS1 mRNA expression in PGF 2α treated FPS cells. (A) FPS cells were treated with vehicle or 100nM PGF2α for 0, 2, 4, 6, 8, 12, 16, 24, 36, 48, 64 and 72 hrs and subjected to quantitative RT-PCR analysis for ADAMTS1. (B) FPS cells were treated for 8hrs with vehicle or 100nM PGF2α in the absence/presence of the FP receptor antagonist (AL8810), Gq/11 inhibitor (YM-254890), calmodulin inhibitor (W7), NFAT inhibitor (INCA6), c-Src inhibitor (PP2), PLC inhibitor (U73122), JNK inhibitor (JNKi) or MEK inhibitor (PD98059) and mRNA expression of ADAMTS1 was determined by quantitative RT-PCR analysis. * represents statistical significance; P < 0.05. Data are represented as mean ± SEM from at least 3 independent experiments.