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Table 3 Summary of CIZ1 alternative splicing in TTC466 Ewing cell line

From: Differential detection of alternatively spliced variants of Ciz1 in normal and cancer cells using a custom exon-junction microarray

TTC466 exon probes

Average of 3 technical replicates

(Biol. 1)

Biological

replicate 2

Biological

replicate 3

Ciz1-ex1

+

nd

=

Ciz1-ex1b1

+

=

=

Ciz1-ex1c

+

+

+

Ciz1-ex2

+

=

=

Ciz1-ex3

+

+

+

TTC466 junction probes

   

Ciz1-ex1ca1-ex2

+

+

+

Ciz1-ex1c-ex2a2

+

+

+

Ciz1-ex1c-ex4

+

+

+

Ciz1-ex1c-ex6

+

+

+

Ciz1-ex2-ex7-a

+

+

+

Ciz1-ex2-ex7-b

+

=

=

Ciz1-ex4-ex5

+

+

+

Ciz1-ex7-ex10

+

+

+

Ciz1-ex8a0-ex12a1

-

nd

_

Ciz1-ex9-ex10

+

+

+

Ciz1-ex13-ex14

+

=

=

Ciz1-ex15-ex16

_

=

=

Ciz1-ex16-ex17

+

=

=

  1. Three independent RNA isolates from proliferating TTC466 cells (Biological replicates, B1, B2 and B3) were analysed on individual arrays, with one isolate undergoing analysis in triplicate (technical replicate, T1, T2 and T3). Probes that are up or down regulated more that 1.5 fold and who's SEM indicate agreement between three technical replicates are shown. Results for these probes derived from the two further biological replicates (replicate 2 and replicate 3) are also shown. + indicates significantly up-regulated, - indicates significantly down-regulated and = indicate no significant change. For paediatric cancer cell line TTC466, expression of 2 exon probes was consistently and significantly altered compared to control samples, these are Ciz1-ex1c and Ciz1-ex3. For exon junction probes a total of 8 probes met all our selection criteria. These are Ciz1-ex1ca1-ex2, Ciz1-ex1c-ex2a2, Ciz1-ex1c-ex4, Ciz1-ex1c-ex6, Ciz1-ex2-ex7-a, Ciz1-ex4-ex5, Ciz1-ex7-ex10 and Ciz1-ex9-ex10. Raw data is given in Additional file 6, tables S9-S13.