NF-κB functional impairment, in response to 17-AAG administration in bladder cancer cells. (A) Characteristic immunofluorescence images of T24 cells presenting NF-κB (green color) cellular localization, under control (i-iii) or 10 μM of 17-AAG (iv-vi) treatment conditions (scale bars: 10 μm). Nuclear counterstaining was performed by the utilization of propidium iodide, while images were visualized by Confocal microscopy and appropriately merged to reveal NF-κB localization in bladder cancer cells (white arrows). (B) Expression profiles of the two anti-apoptotic NF-κB target genes cIAP1 and Survivin in RT4, RT112 and T24 bladder cancer cells, upon exposure to 17-AAG for 24 hours. All experiments were repeated three times.