17-AAG-induced downregulation of Hsp90 in urinary bladder cancer cells, after 24 hours of drug treatment. (Α) Total and truncated protein levels of critical members of the "chaperosome", such as Hsp90, Hsp70 and CHIP, are visualized by a Western blotting approach. Pan-actin was used as protein of reference, while α-tubulin revealed a similar to Hsp90 expression profile. The transcriptional activity analysis of Hsp90 genes (α and β), in response to 17-AAG, is provided in (B). All experiments were repeated three times.