Skip to main content

Table 1 primers and beacon probes used in this study:

From: Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF) as a reference

gene

Primer forward

Beacon probe

Primer reverse

MGMT METHYLATED

5'-TTTCGACGTTCGTAGGTTTTCG+C-3'

5'-FAM- CCGGAGCGTATCGTTTGCGATTTGGTGAGTGTGCTCCGG-BHQ1-3'

5'-GCACTCTTCCGAAAACGAAAC+G-3'

MGMT UNMETHYLATED

5'-TTTGTGTTTTGATGTTTGTAGGTTTTTG+T-3'

5'-FAM-CCGGTGCTGTATTGTTTGTGATTTGGTGAGTGTGCACCGG-BHQ1-3'

5'-AACTCCACACTCTTCCAAAAACAAAAC+A-3'

SNURF METHYLATED

5'-GGATTTTTGTATTG+CGGTAAATAAGTA+C-3'

5'-FAM- CCGGAGGGAGGTAGGTTGGCGCGTATGTTTAGCCTCCGG-BH1-3'

5'-CGCTACAACAAC+GACAAACTTC+G-3'

SNURF UNMETHYLATED

5'-GGATTTTTGTATTG+TGGTAAATAAGTA+T-3'

5'-FAM- CCGGAGGGAGGTAGGTTGGTGTGTATGTTTAGGCCTCCGG-BH1-3'

5'-CTCACTACAACAAC+AACAAACTTC+A-3'

SNURF FLANKING PRIMERS

GGGAGTT+GGGATTTTT+GTATTG

 

CTCCC+CAAACTATCT+CTTAAAAAAAA

  1. (LNA nucleotides are preceeded by +; CpG discriminatory LNA nucleotides are in bold)