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Table 1 primers and beacon probes used in this study:

From: Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF) as a reference

gene Primer forward Beacon probe Primer reverse
MGMT METHYLATED 5'-TTTCGACGTTCGTAGGTTTTCG+C-3' 5'-FAM- CCGGAGCGTATCGTTTGCGATTTGGTGAGTGTGCTCCGG-BHQ1-3' 5'-GCACTCTTCCGAAAACGAAAC+G-3'
MGMT UNMETHYLATED 5'-TTTGTGTTTTGATGTTTGTAGGTTTTTG+T-3' 5'-FAM-CCGGTGCTGTATTGTTTGTGATTTGGTGAGTGTGCACCGG-BHQ1-3' 5'-AACTCCACACTCTTCCAAAAACAAAAC+A-3'
SNURF METHYLATED 5'-GGATTTTTGTATTG+CGGTAAATAAGTA+C-3' 5'-FAM- CCGGAGGGAGGTAGGTTGGCGCGTATGTTTAGCCTCCGG-BH1-3' 5'-CGCTACAACAAC+GACAAACTTC+G-3'
SNURF UNMETHYLATED 5'-GGATTTTTGTATTG+TGGTAAATAAGTA+T-3' 5'-FAM- CCGGAGGGAGGTAGGTTGGTGTGTATGTTTAGGCCTCCGG-BH1-3' 5'-CTCACTACAACAAC+AACAAACTTC+A-3'
SNURF FLANKING PRIMERS GGGAGTT+GGGATTTTT+GTATTG   CTCCC+CAAACTATCT+CTTAAAAAAAA
  1. (LNA nucleotides are preceeded by +; CpG discriminatory LNA nucleotides are in bold)