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Figure 3 | BMC Cancer

Figure 3

From: Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF) as a reference

Figure 3

Standard curves for MS-qLNAPCR with LNA primers. MS-qLNAPCR standard curves with serial dilution mixtures of SssI-treated DNA:untreated DNA (100%, 50%, 10%, 1%, 0.1%, 0.01%, 0.001%; each containing 1.5 μg of template DNA) for LNA primers specific for mMGMT. LNA modified primers for mMGMT show higher PCR efficiency (slope: -3.271; efficiency: 102%) than conventional primers (slope: -4.339; efficiency: 69%, see Fig.4). The analytical detection limit of 0.01% is reached only with LNA primers.

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BMC Cancer

ISSN: 1471-2407

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