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Figure 1 | BMC Cancer

Figure 1

From: Validation and use of microdialysis for determination of pharmacokinetic properties of the chemotherapeutic agent mitomycin C - an experimental study

Figure 1

In vitro microdialysis of mitomycin C (MMC). a. Comparison of in vitro relative recovery by gain (RRgain) and RRloss. In vitro RRgain and RRloss calculated from experiments with identical concentrations of MMC in Ringer's-Acetate for the individual probe. In each experimental setup 12 replicates were collected from each probe, with one probe for each concentration. The results are presented as mean RR (SD). b. The effect of perfusion flow rate on RR. The effect of perfusion flow rate on RR in three experiments with concentrations of MMC in rat plasma of 1.0, 2.5 and 5.0 μM, with one probe for each concentration. The flow was stepwise reduced from 6 to 0.5 μl/min. RR represent the relation between the dialyste concentration and total concentration of MMC. The results are presented as mean RR (pos.SD). c. In vitro zero-net flux (ZNF). ZNF was performed on MMC in rat plasma at concentrations of 1.0, 2.5 and 5.0 μM, with one probe for each concentration. The concentrations of MMC in perfusate were 0.5, 4 and 10 μM. Mean ΔC (=Cin-Cout) with pos SD calculated from three experiments. The interceptions of the regression lines on the x-axis are equal to the free concentrations of MMC in plasma, while the slopes of the regression lines represent RR for the probes.

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