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Table 3 Immunohistochemical analysis of defined MRP-expressing cell lines*

From: MRP3: a molecular target for human glioblastoma multiforme immunotherapy.

Antibody Parent MRP1-tf MRP2-tf MRP3-tf MRP4-tf MRP5-tf
Negative Control 0 0 0 0 0 0
Anti-MRP1 0 2+ 0 0 0 0
Anti-MRP2 0 0 2+ 0 0 0
Anti-MRP3 (M3II-9) 0 0 0 2+ 0 0
Anti-MRP3 (16A11) 0 0 0 2+ 0 0
Anti-MRP3 (1708) 0 0 0 2+ 0 0
Anti-MRP4 0 0 0 0 2+ 0
Anti-MRP5 0 0 0 0 0 2+
  1. *Parent, untransfected lines and their single MRP-transfected, derived lines (-tf) were grown on Lab-Tek chambers for IHC analysis. Primary antibodies and irrelevant controls were used at a concentration of 12.5 μg/ml (MAbs) or 10 μg/ml (polyvalent serum 1708 or normal rabbit IgG). Columns are headed by the target cell line designation; rows are labeled to the left with the primary antibody used for specific MRP detection. 0, 2+: levels of reactivity of the antibodies with each defined MRP-expressing cell line.