Figure 2

Overexpression of Bmi-1 in ESCC tumors. Eight paired primary esophageal tumors (T) and normal esophageal tissue (N) from the same patient were determined for Bmi-1 expression at protein level by Western blot (A), or at mRNA level by real-time PCR (B). Expression level was normalized by α-Tubulin or GAPDH. Error bars represent standard deviations (SD) calculated from three parallel experiments.