Phenotyping breast cancer cell lines EM-G3, HCC1937, MCF7 and MDA-MB-231 using 2-D electrophoresis and affinity chromatography for glutathione-binding proteins

Table 3 Proteins identified from GSH-binding fractions of breast cell lines

Spot no.	Accession no.	Protein name	Theor. Mr/pI	Meas. Mr/pI	Seq. cov. (%)	Pept. seq.
1	P09211	GST P1-1	23,2/5,4	25/5,5	52	15
2	P09211	GST P1-1	23,2/5,4	25/5,7	47	7
3	P09211	GST P1-1	23,2/5,4	25/5,3	51	10
4	P21266	GST M3-3	26,4/5,4	27/5,6	33	8
5	P24534	EF 1-β	24,6/4,5	30/4,5	24	6
6	P26641	EF 1-γ	50,0/6,3	50/nd^a	18	7
7	P60709	β-actin	41,6/5,3	44/5,3	18	7
8	P16152	Carbonyl reductase [NADPH] 1	30,2/8,5	32/8,5	20	5
9	O43813	LanCL 1	45,3/7,9	40/nd^a	5	2

For each protein spot we show the accession number in the Swiss-Prot database, the protein name or its abbreviation, the theoretical (Theor.) and experimental (Meas.) relative molecular weight (in kDa) and isoelectric point (Mr/pI), the sequence coverage and the number of peptides sequenced. Protein names are abbreviated as GST = glutathione S-transferase, EF = elongation factor, LanCL 1 = LanC-like protein 1.
a) The proteins were identified from 1-D SDS-PAGE gels, and their positions in 2-D gels were estimated according to their Mr/pI (Figure 3).

ISSN: 1471-2407