Figure 2

Stimulation of XBP-1 splicing in Raji and Daudi cells treated with RES. Raji (A) and Daudi (B) cells were incubated with 100 μM of RES for the indicated time, total RNA was extracted form the cells and the mRNA of XBP-1 was detected and analyzed by RT-PCR. The RT-PCR products were analyzed by agarose gel electrophoresis. GAPDH was used as the internal control. C, XBP-1 expression in nuclear lysates was analyzed using Western blot. The cells were treated with either 100 μM of RES or 10 μg/ml of Tm for 8 h. Antibody against histone H2B was used as loading control.