Figure 7
Analysis of TIFs from tumour xenograft tumours. (A) Concentration of murine S100A4 in TIFs measured by mouse-specific sandwich ELISA (** p = 0,001). (B) Gelatine zymography showing upregulation of MMP-2 activity in TIFs obtained from tumours co-injected with HMF3s and MCF7S1 cells. The lower panel shows quantification of the activity of MMP-2 and MMP-9. Notice that only MMP-2 activity is significantly upregulated (p = 0.001). (C) Western blot analysis of TIFs from tumours co-injected with HMF3s and MCF7S1 cells showing upregulation of MMP-2 expression. The lower panel shows quantification of band intensities with a significant increase (p = 0.029). (D) Comparison of human-specific cytokine profiles of TIFs from mono- and co-injection xenografts. Cytokines altered from mono- to co-injections are numbered 1 to 3 (1, bFGF↓; 2, IL-8↓; 3, TIMP-1↑). (D) Comparison of mouse-specific cytokine profiles of TIFs from mono- and co-injection xenografts. Cytokines altered from mono- to co-injections are numbered 4 to 9 (4, IL-6↑; 5, pro-MMP9↓; 6, Resistin↑; 7, Thymus CK-1↓; 8, TIMP-2↑; 9, MMP2↑).