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Figure 6 | BMC Cancer

Figure 6

From: Human mammary fibroblasts stimulate invasion of breast cancer cells in a three-dimensional culture and increase stroma development in mouse xenografts

Figure 6

Immunohistochemical analysis of tumour xenografts. (A) Immunohistochemical staining of tumour xenografts against fibronectin, laminin, S100A4, and F4/80. Green arrows indicate elongated S100A4-positive cells, and red arrows indicate smaller rounded cells. Scale bars = 100 μm. (B) Double-immunostaining of tumour sections from HMF3s + MCF7S1 co-injections detecting S100A4 (green) and human prolyl-4-hydroxylase (red). Yellow arrows indicate elongated cells expressing S100A4 but not prolyl-4-hydroxylase. Scale bar = 100 μm. (C) Positive control for prolyl-4-hydroxylase staining on cell cultures of the HMF3s fibroblast (red = prolyl-4-hydr, S100A4 = green, scale bar = 200 μm) and a human tumour sample (scale bar = 50 μm).

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