Figure 6

Histone acetylation regulates leptin expression in HepG2 cells. (a) TSA treatment (200, 500, 1000 nM) of HepG2 cells for 24 h increased leptin's mRNA and protein expression. Columns, means of three independent experiments done in triplicate; bars, SD, *, p < 0.001 compared to untreated cells, (b) After TSA treatment H3 was found acetylated in proximal leptin promoter.