Skip to main content

Table 4 DEF, CI, ID50, SF2 and % survival (representing the cytotoxic effect of treatment with MTA and/or dFdC alone, 0 Gy) for MTA - dFdC - irradiation (RT) combinations in CAL-27 and A549 cells.

From: In vitro study on the schedule-dependency of the interaction between pemetrexed, gemcitabine and irradiation in non-small cell lung cancer and head and neck cancer cells

  Condition DEF CI ID50 SF2 % survival
   mean SD   mean SD   mean SD   mean SD   mean SD  
CAL-27 RT        1.94 0.47   48.37 9.31   100% 0%  
  24 h 50 nM MTA → RT 1.04 0.23   1.095 0.127   1.97 0.45   49.25 9.71   97% 11%  
  24 h 50 nM MTA → 24 h → RT 1.59 0.53   0.916 0.063   1.71 0.66   43.37 12.35   70% 21%  
  24 h 50 nM MTA → 1 h → RT 1.46 0.21   0.933 0.073   1.21 0.35   32.45 8.43   98% 4%  
  24 h 2 nM dFdC → RT 1.47 0.09   0.888 0.180 * 1.72 0.10   49.25 9.71   96% 1%  
  24 h 2 nM dFdC → 24 h → RT 1.04 0.16   1.090 0.101   2.43 0.08   58.31 2.96   84% 18%  
  1 h 100 nM dFdC → 24 h → RT 1.56 0.46   0.653 0.108 ** 1.32 0.25   34.09 5.34   91% 24%  
  1 h 50 nM dFdC → RT 1.07 0.19   0.917 0.124   1.69 0.64   44.73 14.77   98% 14%  
  24 h 2 nM dFdC + 24 h 50 nM MTA → RT 1.53 0.27 0.960 0.267   1.66 0.09   42.27 1.03   98% 11%  
  24 h 2 nM dFdC → 24 h 50 nM MTA → RT 1.38 0.09   0.949 0.106   1.85 0.34   46.93 6.81   75% 4%
  24 h 50 nM MTA → 24 h 2 nM dFdC → RT 1.78 0.14   0.705 0.097 * 1.42 0.06   36.83 3.36   64% 22%  
  1 h 100 nM dFdC → 24 h 50 nM MTA → RT 1.56 0.37 0.801 0.094 * 1.29 0.20 34.65 4.07 71% 15%
  24 h 50 nM MTA → 1 h 50 nM dFdC → RT 1.43 0.57   0.858 0.175 * 1.30 0.45   34.93 10.25   65% 11% †;††
A549 RT        2.41 0.39   56.26 6.18   100% 0%  
  24 h 100 nM MTA → RT 1.29 0.25   0.674 0.053 ** 1.77 0.36   45.39 7.15   87% 11%  
  24 h 100 nM MTA → 24 h → RT 0.98 0.15   0.838 0.070 * 2.16 0.11   52.70 2.00   84% 16%  
  24 h 100 nM MTA → 1 h → RT 1.52 0.39   0.681 0.084 ** 1.85 0.51   46.28 9.78   85% 11%  
  24 h 2 nM dFdC → RT 1.55 0.24   0.538 0.057 ** 1.25 0.08   34.55 2.01   71% 16%  
  24 h 2 nM dFdC → 24 h → RT 0.99 0.31   0.703 0.118 * 2.04 0.40   50.19 6.96   65% 4%  
  1 h 100 nM dFdC → 24 h → RT 1.23 0.23   0.606 0.073 ** 1.97 0.24   49.28 4.36   67% 13%  
  1 h 50 nM dFdC → RT 1.49 0.39   0.831 0.068 * 1.92 0.49   47.99 8.72   96% 5%  
  24 h 2 nM dFdC + 24 h 100 nM MTA → RT 1.49 0.28   0.564 0.075 ** 1.32 0.30   36.11 6.47   66% 23%  
  24 h 2 nM dFdC → 24 h 100 nM MTA → RT 0.94 0.27   0.585 0.101 ** 2.21 0.80   52.39 11.98   47% 14%  
  24 h 100 nM MTA → 24 h 2 nM dFdC → RT 1.17 0.24   0.500 0.073 ** 1.69 0.36   44.34 7.16   48% 16%
  1 h 100 nM dFdC → 24 h 100 nM MTA → RT 1.29 0.27   0.513 0.053 ** 1.88 0.25   47.68 4.45   48% 12%
  24 h 100 nM MTA → 1 h 50 nM dFdC → RT 1.52 0.58   0.534 0.083 ** 1.92 0.50   47.93 9.71   49% 8% †;††
  1. †: p < 0.05 vs. corresponding schedule of MTA and RT; ††: p < 0.05 vs. corresponding schedule of dFdC and RT; *: moderate synergism (0.7<CI<0.9); **: synergism (CI<0.7).