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Table 1 DEF, CI, ID50, SF2 and % survival (representing the cytotoxic effect of treatment with MTA alone, 0 Gy) for irradiation (RT) given alone or in combination with MTA using different treatment schedules: 24 h MTA immediately followed by RT; RT immediately followed by 24 h MTA; 24 h MTA followed by different time intervals (24, 8, 4, 1, 0 h) and subsequent irradiation.

From: In vitro study on the schedule-dependency of the interaction between pemetrexed, gemcitabine and irradiation in non-small cell lung cancer and head and neck cancer cells

  Condition DEF CI ID50 SF2 % survival
   mean SD   mean SD   mean SD   mean SD   mean SD
CAL-27 RT        1.94 0.47   48.37 9.31   100% 0%
  24 h 50 nM MTA → RT 1.04 0.23   1.145 0.132   1.97 0.45   49.25 9.71   97% 11%
  24 h 75 nM MTA → RT 1.01 0.10   1.260 0.270   1.94 0.54   47.75 7.53   70% 12%
  24 h 100 nM MTA → RT 1.04 0.03   1.229 0.269   1.86 0.27   47.34 4.31   57% 4%
  RT → 24 h 50 nM MTA 0.87 0.06   1.039 0.098   1.86 0.46   46.21 9.19   88% 4%
  RT → 24 h 75 nM MTA 0.94 0.14   1.023 0.063   1.77 0.63   44.56 12.48   71% 8%
  RT → 24 h 100 nM MTA 1.00 0.24   1.005 0.080   1.65 0.41   42.40 10.00   59% 12%
  24 h 50 nM MTA → 24 h → RT 1.59 0.53 0.915 0.063   1.71 0.66   43.37 12.35   70% 21%
  24 h 50 nM MTA → 8 h → RT 1.23 0.31   0.844 0.091 * 1.55 0.58   39.45 12.88   88% 4%
  24 h 50 nM MTA → 4 h → RT 1.12 0.24   0.965 0.106   1.68 0.58   42.34 12.02   90% 6%
  24 h 50 nM MTA → 1 h → RT 1.46 0.21 0.932 0.073   1.21 0.35 32.45 8.43 98% 4%
A549 RT        2.41 0.39   56.26 6.18   100% 0%
  24 h 100 nM MTA → RT 1.29 0.25   0.698 0.079 ** 1.77 0.36   45.39 7.15   87% 11%
  24 h 200 nM MTA → RT 1.19 0.06   0.855 0.197 * 2.18 0.34   52.16 4.46   65% 12%
  24 h 300 nM MTA → RT 0.97 0.23   0.887 0.294 * 2.84 1.03   57.46 8.70   52% 14%
  24 h 500 nM MTA → RT 0.75 0.28   0.793 0.288 * 3.83 1.56   62.76 10.23   38% 13%
  RT → 24 h 200 nM MTA 0.86 0.19   0.996 0.124   2.77 0.20   60.24 3.01   68% 19%
  RT → 24 h 300 nM MTA 0.80 0.10   0.862 0.173 * 2.98 0.30   61.48 2.65   52% 16%
  RT → 24 h 500 nM MTA 0.76 0.11   0.723 0.166 * 3.11 0.19   62.75 1.09   38% 15%
  24 h 100 nM MTA → 24 h → RT 0.98 0.15   0.832 0.067 * 2.16 0.11   52.70 2.00   84% 16%
  24 h 100 nM MTA → 8 h → RT 1.21 0.18   0.731 0.129 * 2.02 0.24   50.62 4.87   85% 9%
  24 h 100 nM MTA → 4 h → RT 1.56 0.41   0.718 0.103 * 1.63 0.35   42.19 6.30   94% 10%
  24 h 100 nM MTA → 1 h → RT 1.52 0.39   0.677 0.076 ** 1.85 0.51   46.28 9.78   85% 11%
  1. †: p < 0.05 vs. 24 h incubation with the same concentration of MTA immediately followed by RT; *: moderate synergism (0.7<CI<0.9); **: synergism (CI<0.7).