Reduced levels of CK2 affect the pro-survival PI3K/AKT and mTOR signaling pathways in PANC-1 cells. A. Western blot analysis of mTOR signaling pathway-related proteins in PANC-1 cells treated as indicated in the figure. B. Total lysates (80 μg) from cells treated as shown in the figure, were subjected to Western blot analysis with the indicated antibodies against PI3K/AKT signaling pathway-related proteins. β-actin was used as control for equal loading. C. Whole lysates from cells treated as indicated in the figure were subjected to Western blot analysis with the indicated antibodies. Data shown are representative of three independent experiments.