Apoptotic cell death induced by 5-FU, LY294002, or their combination. (A) Morphological changes were induced in SNU-719 cells by combined treatment with 5-FU and LY294002. Nuclear fragmentation and condensation were evident in the cells treated with the sequential combination of 5-FU and LY294002. (B) Flow-cytometric analysis of apoptosis was performed. The untreated cells and cells treated with the experimental drugs were stained with propidium iodide and annexin V. The values are the averages of three independent experiments (mean ± SD).