Table 2 Summary of primer sequences*, annealing temperatures, and PCR product sizes used for MSP
Gene | Forward primer (5' - 3') | Reverse primer (3' - 5') | Tm (°C) | Size (bp) |
|---|---|---|---|---|
p16 | M: TTATTAGAGGGTGGGGCGGATCGC** | M: GACCCCGAACCGCGACCGTAA | 60 | 150 |
U: TTATTAGAGGGTGGGGTGGATTGT | U: CAACCCCAAACCACAACCATAA | 60 | 151 | |
DAPK | M: GGATAGTCGGATCGAGTTAACGTC | M: CCCTCCCAAACGCCGA | 59 | 98 |
U: GGAGGATAGTTGGATTGAGTTAATGTT | U: CAAATCCCTCCCAAACACCAA | 59 | 106 | |
RARβ | M: TCGAGAACGCGAGCGATTCG | M: GACCAATCCAACCGAAACGA | 56 | 146 |
U: TTGAGAATGTGAGTGATTTGA | U: AACCAATCCAACCAAAACAA | 56 | 146 |