Table 1 Summary of primer sequences, annealing temperatures and PCR product sizes used for CYP1A1 (MspI) and GSTM1
Gene | Primer | °C | bp |
|---|---|---|---|
CYP1A1 (MspI) | Forward 5'-CAGTGAAGAGGTGTAGCCGCT-3' | 60 | 340 |
Reverse 5'-TAGGAGTCTTGTCTCATGCCT-3' | |||
GSTM1 | Forward 5'-GAACTCCCTGAAAAGCTAAAGC-3' | 59 | 215 |
Reverse 5'-GTTGGGCTCAAATATACGGTG G-3' | |||
β-Globin (reference) | Forward 5'-CAACTTCATCCACGTTCACC-3' | 59 | 268 |
Reverse 5'-GAAGAGCCAAGGACAGGTA-3' |