Figure 5

Apoptosis induction by survivin knockdown and MSA treatment in PC-3M cells. Cells were transfected with the shRNAs and treated with 5 μM MSA for 66 hr. A, Quantitation of apoptotic cells by PI staining and flow cytometry analysis. Apoptosis index was calculated as percentage of cells in the sub-G1 population on the DNA histogram. The data presented are mean ± SD (n = 3). The values are 0.9% ± 0.65%, 2.8% ± 0.71%, 23.1 ± 2.36%, 12.7 ± 1.45%, and 38.6 ± 2.87%, respectively. B, Western blot analyses of caspase-3. β-actin was used as the loading control. B. Quantitation of cleaved caspase-3 by volume densitometry. The intensity of the cleaved caspase-3 band was quantified by volume densitometry and normalized to that of β-actin. The result was expressed as fold to control.