Figure 2

Salivary gland dysfunction is reversed in mice receiving post therapy IGF-1. The head and neck region of FVB mice was exposed to a single 5 Gy radiation dose and mice received injections of IGF-1 or vehicle on days 4-8 as depicted in figure 1A. Stimulated salivary flow rates were determined as described in the materials and methods section on days 30 (A), 60 (B), and 90 (C). Irradiated flow rates were normalized to corresponding unirradiated controls after the radiation treatment. The graph represents the mean and standard error of the mean of all data from 10-23 mice per treatment group. Significant differences (p < 0.05) were determined using an ANOVA followed by a post-hoc Bonferroni multiple-comparison test. Treatment groups with the same letters are not significantly different from each other within the same day.