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Figure 7 | BMC Cancer

Figure 7

From: Nuclear β-catenin and CD44 upregulation characterize invasive cell populations in non-aggressive MCF-7 breast cancer cells

Figure 7

Effects of anti-CD44 antibody treatment on cell proliferation, migration and invasion. (A) Proliferation rates of untreated cells in culture. Data are the means ± SD (n = 3). Statistical analysis was performed by ANOVA and Tukey-Kramer multiple comparison. *, P < 0.01, compared with MCF-7 cells. (B) Proliferation rates of normal rat IgG-treated and anti-CD44 antibody-treated cells. In each cell line, the number of normal IgG-treated cells at 96 h was considered 100%. Data are the means ± SD (n = 3). (C) Optical microscopic images of cell migration assay at 72 h after removal of the stopper. Cells were fixed and stained with crystal violet. (D) Cell migration rates were expressed as percent closure of the open area by comparing the zero time. Data are the means ± SD (n = 6). Statistical analysis was performed by Student's t test or ANOVA/Tukey-Kramer multiple comparison, where appropriate. *, P < 0.01, compared with normal IgG-treated cells., P < 0.01, compared with MCF-7.#, P < 0.01, compared with MCF-7-14 and its clone CL6. (E) Matrigel invasion assay of normal rat IgG-treated and anti-CD44 antibody-treated cells. After 60 h cultivation, cells invading Matrigel were fixed and stained on the underside of the chamber. (F) The numbers of invading cells were counted under a microscope. Data are the means ± SD (n = 3). Statistical analysis was performed by Student's t test or ANOVA/Tukey-Kramer multiple comparison, where appropriate. *, P < 0.01, compared with normal IgG-treated cells., P < 0.01, compared with MCF-7.#, P < 0.01, compared with MCF-7-14 and MDA-MB-231.

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