Figure 3
From: SLUG/SNAI2 and Tumor Necrosis Factor Generate Breast Cells With CD44+/CD24- Phenotype
Signaling network involving CD44+/CD24-enriched EMT-associated genes. A) Ingenuity pathway analysis of EMT-associated genes upregulated in CD44+/CD24- cells compared to CD44-/CD24+ cells. Genes in shaded shapes correspond to genes that are upregulated in CD44+/CD24- cells compared to CD44-/CD24+ cells. B) NF-κB:DNA complexes in unsorted MCF-10A, CD44+/CD24-, and CD44-/CD24+ subpopulation of cells. CD44+/CD24- cells contained higher levels of p65:p50 heterodimers compared to other cell types. SS = supershift. EMSAs with Oct-1 and SP-1 probes are shown as controls.