Western blot and RT-PCR analysis of genes differentially expressed in CD44+/CD24-, CD44-/CD24+ and CD44+/CD24+ cells. A) CD44+/CD24- cells express higher levels of ST-2, N-cadherin, ETV5, FHL-1, Wnt5B, FOXC2, SATB-1, SLUG, and Gli-2, whereas CD44-/CD24+ cells express higher levels of E-cadherin, ST14, ESE1, and KLK7. Western blotting or RT-PCR was performed to measure protein (left panel) or RNA (central panel) from two independent samples (labeled 1 and 2). B) Cell lines with CD44+/CD24- subpopulation express higher levels of SLUG and Gli-2. C) Expression levels of EMT markers and few additional genes in unsorted MCF-10A, CD44+/CD24-, CD44-/CD24+, and CD44+/CD24+ cells. RT-PCR was used to measure SLUG, Gli-2, ESE1, and Bmi-1, whereas western blotting was used to measure E-cadherin, N-cadherin, vimentin, and αSMA.