Figure 4
From: Orphan receptor GPR110, an oncogene overexpressed in lung and prostate cancer
Isoforms 2 and 3 are on the cell surface. Western blots of fractions of surface biotinylated HEK cells transfected with C-terminal HA tagged isoforms 2 (Iso2) and 3 (Iso3), developed with antibody to GAPDH (first blot; arrow points to GAPDH), to integrin β1 (second blot; arrow points to integrin β1), and HA.11 antibody (third blot), respectively. pcDNA3.1, empty vector; L, lysate; U, unbound, and B, bound cell surface fraction.