Figure 4

Adipocyte structure is conserved in tumors from mice treated with GU81 and doxorubicin. A) Sections of tumors from each treatment group were stained by immunofluorescence for perilipin (red), an adipocyte marker, and nuclei (blue). Images representative of each group are shown. A minimum of 3 tumors from each group were evaluated by immunofluorescence. Total magnification, 200×. B&C) Staining from a minimum of 5 images from each tumor per treatment group was quantified by evaluating the Cy3 positive area (perilipin(B) adiponectin (C)). There is significantly more perilipin (B) staining in tumors from mice treated with GU81 + doxorubicin than from tumors treated with either doxorubicin or GU81 alone (p≤0.05 by Mann-Whitney). Adiponectin (C) staining was significantly increased in tumors from mice treated with either doxorubicin alone or GU81 and doxorubicin compared to tumors from control-treated animals (p≤0.05, t-test).