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Figure 3 | BMC Cancer

Figure 3

From: Characterisation of prostate cancer lesions in heterozygous Men1 mutant mice

Figure 3

Prostate cancers from Men1 +/- mice do not express p63 and display heterogeneous AR expression. (A-H) Double IF staining using antibodies against menin (red) and p63 (green) performed on a normal prostate from a 24-month-old Men1 +/+ mouse (A-D, n = 2) and an adenocarcinoma found in a Men1 +/- mouse of the same age (E-H, n = 2). DAPI stains cell nuclei. Note that normal p63-expressing basal cells scattered within the lesion remain menin-positive (shown by white arrowheads). Boxed areas are magnified in the insets. Scale bars, 50 μm. (I-N) IHC using antibody against AR was performed on prostate tissues from Men1 +/+ (I, L, n = 4) and Men1 +/- (J, M and K, N, n = 4) mice. AR expression is present but more heterogeneous in an in situ prostate carcinoma (J, M, 26-month-old mouse) and an adenocarcinoma (K, N, 23-month-old mouse) from Men1 +/- mice, with some cells showing greatly reduced AR expression compared to the control. Panels L-N are two-fold magnifications of the upper panels (I-K). Insets show an amplified view of a part of the prostate glands. (O) Prostatic epithelial nuclei showing absent or very low AR expression were counted in six random microscopic fields in normal prostate glands from Men1 +/+ mice (n = 3) and carcinomas observed in Men1 +/- prostate glands (n = 4), stained with an anti-AR antibody. The results are expressed as a percentage of total prostatic epithelial cells (250 nuclei counts). Values are means ± standard error of the mean. *** Significant difference from normal Men1 +/+ prostate with a two-tailed Student's t-test (P < 0.0001).

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