Figure 2

Cinnamon extract treatment induces active cell death in melanoma cells. At the indicated time periods after treatment of cinnamon extract, cells were stained with Annexin V and 7-ADD. Double positive (Annexin V⁺ and 7-ADD⁺) (A) or Annexin V⁺ positive (B) populations were analyzed by FACS. Gene expression (C) and protein (D) level of pro-apoptotic molecules such as Bad, Bim, Bax and Bak were measured by quantitative real-time PCR or immunoblotting, respectively. Error bars indicated SD. One (*), two (**) or three asterisks (***) indicate p < 0.05, p < 0.005 or p < 0.001, respectively. Data are representative of three independent experiments.