Figure 4
From: Degradation of HER2/neu by ANT2 shRNA suppresses migration and invasiveness of breast cancer cells
ANT2 shRNA down-regulates MT1-MMP expression by suppressing PI3K signaling in SK-BR3 cells (A). Cells were transfected with scramble shRNA or ANT2 shRNA. After 24 hours of incubation, total RNA and cell extracts were prepared for RT-PCR using specific primers for human MT1-MMP or GAPDH (internal control) and western blotting with anti-MT1-MMP and anti-β-actin antibodies. (B) Cells were transfected with scramble shRNA or ANT2 shRNA with or without the wild-type PI3K expression vector and cultured for 24 hours. After 24 hours of incubation, total RNA and cell extracts were prepared for RT-PCR using specific primers for human MT1-MMP or GAPDH (internal control) and western blotting with anti-MT1-MMP and anti-β-actin antibodies.