Down-regulation of HER2/ neu by ANT2 shRNA in HER2/ neu overexpressing breast cancer cell lines (SK-BR3). (A) After 24 hours of transfection with scramble shRNA or ANT2 shRNA, expression levels of HER2/neu were analyzed using flow cytometry. (B) For identification of dissociation and degradation of HSP90 from HER2/neu by ANT2 shRNA, cells were transfected with ANT2 shRNA for 24 hours and the cellular extracts were prepared for immuno-precipitation with anti-Her2/neu antibody and subjected to western blotting with anti-HSP90 and anti-ubiquitin antibodies. HSP90 inhibitor, 17-AAG was used as a positive control. (C) After 24 hours of transfection with ANT2 shRNA, cells extracts were prepared for western blotting with anti-Her2/neu and anti-β-actin antibodies.