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Figure 5 | BMC Cancer

Figure 5

From: Troglitazone suppresses telomerase activity independently of PPARγ in estrogen-receptor negative breast cancer cells

Figure 5

Troglitazone does not induce cell differentiation. MDA-MB-231 cells were treated with 20 μM of troglitazone (Tro 20 μM) or the equal volume of DMSO for 24 hours. A) Representative western blot showing the effect of 20 μM of troglitazone on the protein level of maspin. Western blots of total protein were probed with anti-maspin antibodies and then reprobed with anti-GAPDH to confirm equal loading. B and C) Total RNA from treated and control cells was used to examine the expression level of B) Mucin-1 (Muc-1) and C) Keratin 19 (K19) by real-time RT-PCR. Values express the relative quantity of the genes to the level of mRNA expression of GAPDH. Data shown is representative of three separate experiments.

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