Figure 1

The expression of PPARγ, hTERT and telomerase activity in several breast cancer cell lines. A) Total RNA was prepared from MDA-MB-231, MCF-7, and T47D cells. Using real-time RT-PCR, the mRNA expression of PPARγ was measured and MDA-MB-231 cells had the highest expression of PPARγ mRNA. B) Protein lysates were prepared from the breast cancer cell lines and analyzed via western blotting to examine the level of PPARγ protein. MDA-MB-231 cells express more PPARγ protein than MCF-7 and T47D cells in this representative western blot. C) Total RNA was used to examine the mRNA expression level of hTERT in MDA-MB-231, MCF-7, and T47D cells by real-time RT-PCR. Values express the relative quantity of the genes to the level of mRNA expression of GAPDH. D) Telomerase activity was measured from protein lysates (500 and 250 ng respectively) in MDA-MB-231, MCF-7, and T47D cell lines. I.C. the internal PCR amplification control. CHAPS, lysis buffer only as a negative control. HeLa, 500 cell equivalent lysate as a positive control.