Figure 4

Histone H4 Acetylation by Western Blotting. Fresh cultured Kasumi -1 cells were collected and re-suspended in RPMI 1640 medium with 10% fetal bovine serum in the presence or absence of indicated doses of sodium butyrate for 22 hours. Cells were then collected and lysed. Proteins were subjected to SDS-PAGE and immunoblotted with anti-acetyl-H4. The membrane was stripped and reprobed with anti-beta actin antibody. Immunoblotting demonstrated an increasing pattern of histone H4 acetylation with increasing concentrations of sodium butyrate