Expression of candidate genes in human melanoma cell lines. a, Realtime PCR was performed to determine FOSL1, EGR1, OPN, IGFBP3, DUSP4 and TAAL6 expression in human melanoma cell lines compared to NHEM cells. Fold change of transcript, referred to NHEM cells, is indicated. β-actin was used as reference gene. Significant (p < 0.05) or highly significant (p < 0.001) regulation are marked by one or two asterisks, respectively (Student's t-test, paired, one-tailed).
b, Western blot analysis of the candidate genes in human melanoma cell lines. Expression of ERK2 served as loading control. Please note that the order of cell lines differs from A. On the OPN blot, the lower band represents the OPN protein. For TAAL6, the upper band corresponds to the protein of the expected size.