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Table 2 Effects of transfections on apoptosis induction

From: Simultaneous gene silencing of Bcl-2, XIAP and Survivin re-sensitizes pancreatic cancer cells towards apoptosis

Target gene

 

MiaPaCa-2

AsPC-1

  

mean (S.E.)

mean (S.E.)

Control

Annexin V

3.73 (± 0.5481)

2.23 (± 0.2232)

 

Caspase

2.15 (± 0.2665)

1.17 (± 0.3060)

 

Viable Cells

1.65 (± 0.2651)

1.25 (± 0.1784)

SGS

Annexin V

12.81 (± 2.0870)

4.49 (± 0.2966)

 

Caspase

9.96 (± 1.7829)

9.90 (± 1.9189)

 

Viable Cells

6.70 (± 2.2837)

4.75 (± 0.9908)

XIAP (low ST)

Annexin V

3.96 (± 0.6850)

2.34 (± 0.1797)

 

Caspase

2.41 (± 0.5015)

2.24 (± 1.0513)

 

Viable Cells

2.42 (± 1.0029)

1.20 (± 0.1452)

Survivin (low ST)

Annexin V

5.81 (± 1.0148)

3.17 (± 0.1986)

 

Caspase

3.77 (± 0.5577)

2.77 (± 0.8111)

 

Viable Cells

4.02 (± 0.8603)

2.18 (± 0.1375)

Bcl-2 (low ST)

Annexin V

3.40 (± 0.4986)

2.36 (± 0.1309)

 

Caspase

1.88 (± 0.2001)

1.56 (± 0.4392)

 

Viable Cells

2.27 (± 0.6989)

1.25 (± 0.1200)

XIAP (ST)

Annexin V

4.76 (± 0.7350)

3.19 (± 0.1122)

 

Caspase

7.00 (± 0.3533)

2.55 (± 0.4072)

 

Viable Cells

2.59 (± 0.7848)

1.87 (± 2.3098)

Survivin (ST)

Annexin V

7.77 (± 1.8167)

3.94 (± 0.2719)

 

Caspase

8.40 (± 3.1037)

3.27 (± 0.9557)

 

Viable Cells

3.40 (± 0.7485)

5.23 (± 2.3098)

Bcl-2 (ST)

Annexin V

3.51 (± 0.5311)

2.84 (± 0.1863)

 

Caspase

2.94 (± 0.8894)

2.53 (± 1.1196)

 

Viable Cells

1.49 (± 0.4988)

1.28 (± 0.1331)

  1. Apoptosis induction was analyzed by Annexin V positivity, caspase activation and viable cell count. All results are normalized to non-treated cells. Viable cell counts are depicted in inverse ratios for better comparison. Significant results are marked in bold.
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BMC Cancer

ISSN: 1471-2407

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