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Table 2 Effects of transfections on apoptosis induction

From: Simultaneous gene silencing of Bcl-2, XIAP and Survivin re-sensitizes pancreatic cancer cells towards apoptosis

Target gene   MiaPaCa-2 AsPC-1
   mean (S.E.) mean (S.E.)
Control Annexin V 3.73 (± 0.5481) 2.23 (± 0.2232)
  Caspase 2.15 (± 0.2665) 1.17 (± 0.3060)
  Viable Cells 1.65 (± 0.2651) 1.25 (± 0.1784)
SGS Annexin V 12.81 (± 2.0870) 4.49 (± 0.2966)
  Caspase 9.96 (± 1.7829) 9.90 (± 1.9189)
  Viable Cells 6.70 (± 2.2837) 4.75 (± 0.9908)
XIAP (low ST) Annexin V 3.96 (± 0.6850) 2.34 (± 0.1797)
  Caspase 2.41 (± 0.5015) 2.24 (± 1.0513)
  Viable Cells 2.42 (± 1.0029) 1.20 (± 0.1452)
Survivin (low ST) Annexin V 5.81 (± 1.0148) 3.17 (± 0.1986)
  Caspase 3.77 (± 0.5577) 2.77 (± 0.8111)
  Viable Cells 4.02 (± 0.8603) 2.18 (± 0.1375)
Bcl-2 (low ST) Annexin V 3.40 (± 0.4986) 2.36 (± 0.1309)
  Caspase 1.88 (± 0.2001) 1.56 (± 0.4392)
  Viable Cells 2.27 (± 0.6989) 1.25 (± 0.1200)
XIAP (ST) Annexin V 4.76 (± 0.7350) 3.19 (± 0.1122)
  Caspase 7.00 (± 0.3533) 2.55 (± 0.4072)
  Viable Cells 2.59 (± 0.7848) 1.87 (± 2.3098)
Survivin (ST) Annexin V 7.77 (± 1.8167) 3.94 (± 0.2719)
  Caspase 8.40 (± 3.1037) 3.27 (± 0.9557)
  Viable Cells 3.40 (± 0.7485) 5.23 (± 2.3098)
Bcl-2 (ST) Annexin V 3.51 (± 0.5311) 2.84 (± 0.1863)
  Caspase 2.94 (± 0.8894) 2.53 (± 1.1196)
  Viable Cells 1.49 (± 0.4988) 1.28 (± 0.1331)
  1. Apoptosis induction was analyzed by Annexin V positivity, caspase activation and viable cell count. All results are normalized to non-treated cells. Viable cell counts are depicted in inverse ratios for better comparison. Significant results are marked in bold.