Relationship between EGFR expression, copy number and mutation in lung adenocarcinomas

BMC Cancer

Table 3 Relationship between EGFR protein expression and EGFR copy number, and mutation

IHC criteria	Mutation			FISH
	positive	negative	P value	positive	negative	P value
First criteria
Positive (>10% tumor cells, ++ and +++)	65	26	0.008	45	46	0.012
Negative	20	22		11	31
Second criteria
Positive (>10% tumor cells, any intensity)	76	38	0.105	51	63	0.132
Negative	9	10		5	14
Third criteria Positive (H Score ≥ 100)	62	24	0.008	42	44	0.027
Negative(H Score < 100)	23	24		14	33

After Bonferroni correction, P < 0.025 was considered as significant. For using the first IHC criteria, IHC were significantly associated with mutation and FISH. For using the second IHC criteria, no significant association was found between IHC and mutation or FISH. For using the third IHC criteria, IHC was significantly associated with mutation, approached significant associated with FISH(P = 0.027,close to 0.025)

ISSN: 1471-2407