Table 1 Modifications of chemokines induced by coculturing Mφ with MDA-MB-231 cells
Chemokine | Expressed in | Overexpressed in coculture | Family | Function | |
|---|---|---|---|---|---|
|  | Mφ | MDA |  |  |  |
ENA 78 (CXCL5) | - | - | + | CXC chemokine with ELR motif | - Pro-angiogenic activity - Neutrophil chemotaxis - Monocyte migration |
GM-CSF (CSF2) | - | + | + | Growth factor | - Stimulates stem cells to produce granulocytes and monocytes - Enhances monocytic migration via RhoA and integrin activation, and via MMP expression |
GRO (CXCL1, 2, 3) | + | + | + | CXC chemokine with ELR motif | - Angiogenic activity - Increased migration of PBMC/monocytes |
GROα (CXCL1) | - | + | + | CXC chemokine with ELR motif | - Angiogenic activity - Increased migration of PBMC/monocytes |
IL-6 | - | + | + | Inflammatory cytokine | - Inflammatory cytokine with a well-documented role in cancer - Recruitment of myelo-monocytes |
IL-8 (CXCL8) | + | + | + | CXC chemokine with ELR motif | - Migration of neutrophils - Important role in tumour growth, angiogenesis, and metastasis |
IL-10 | - | - | + | - Anti-inflammatory cytokine | - Anti-inflammatory cytokine produced by M2 Mφ |
MCP-1 (CCL2) | + | - | + | CC chemokine | - Recruitment of monocytes to sites of injury |
MCP-3 (CCL7) | - | - | + | CC chemokine | - Inflammatory cytokine |
MCSF (CSF1) | - | - | + | Growth factor | - Facilitates monocyte survival, monocyte-to- Mφ conversion, Mφ proliferation, M1 to M2 switch |
MDC (CCL22) | - | - | + | CC chemokine | -Chemotactic for monocytes |