Figure 5

IL-10 secretion by Mφ cultured in the absence or presence of MDA-MB-231. Cells were incubated for 5 days in the absence (A) or presence (B) of TM (5 μM, final concentration), the corresponding medium being renewed daily; then culture media corresponding to the final 24 h of incubation were tested for IL-10 by ELISA. No IL-10 was detected in medium from MDA-MB-231 cultured alone. Mean ± SEM of 3 independent experiments. * p < 0.05 between Mφ without TM and coculture without TM; p < 0.05 between Mφ without TM and Mφ with TM.