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Figure 4 | BMC Cancer

Figure 4

From: Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate

Figure 4

Apoptosis of Mφ, MDA-MB-231 breast cancer cells, and coculture cells. Cells were incubated for 5 days in the absence (A) or presence (B) of TM (5 μM, final concentration), the corresponding medium being renewed daily. Cell Death Detection ELISA PLUS enzyme immunoassay (Roche Applied Science) was used for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) in the cytoplasmic fractions of cells from these 5-day cultures. The results are expressed as the absorbance at 405 nm minus the absorbance at 492 nm. The apoptosis index measured in the coculture was equivalent to the sum of the index measured in cultures of MDA-MB-231 alone plus that of Mφ alone. Mean ± SEM of 3 independent experiments.

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