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Figure 1 | BMC Cancer

Figure 1

From: Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate

Figure 1

Effect of the cooperation between Mφ and MDA-MB-231 cells on vascularisation of the CAM. MDA-MB-231 cells were seeded at a density of 7.8 × 104 cells/cm2, with (coculture) or without the same number of Mφ. Spent medium was replaced by fresh medium every day. After 5 days of incubation, the conditioned media corresponding to the final 24 h of culture were tested for their angiogenic activity as follows: each day, for 3 days, 33 μL of these media were introduced into silicon rings placed on CAMs from 6-day-old chick embryos. bFGF (25 ng/ml) was used as positive control. Panel A: Treated areas were photographed 24 h after the last treatment. Note the increase in the CAM of a denser secondary capillary network induced by the coculture medium, in comparison to that induced by conditioned medium from either MDA-MB-231 or Mφ cultured alone. This secondary capillary network was inhibited by the addition of 5 μM TM during the coculture. Panel B: total number of vessels that sprouted from the primary vessels of the CAM and total length of the neoangiogenesis. Mean of 3 independent experiments ± SEM. * p < 0.05, Mann-Whitney U test.

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