Figure 2
From: Benign mammary epithelial cells enhance the transformed phenotype of human breast cancer cells
G-12A and HuMEC benign mammary epithelial cells enhance the anchorage-independent growth of R2-T1AS breast cancer cells in soft agar. (A) Soft agar co-culture of R2-T1AS cells and G-12A cells. R2-T1AS cells were plated alone at a density of 1 × 104 cells per well (denoted as 1, red bar), in the R2-T1AS/G-12A co-culture, 1 × 104 R2-T1AS were mixed with 8 × 104 G-12A cells (denoted as 8, purple stippled bar), and in the R2-T1AS/G2B-10A co-culture 1 × 104 R2-T1AS were mixed with 8 × 104 G2B-10A cells (denoted as 8, red stippled bar). Graph shows mean colony numbers (± standard error) obtained in 3 independent experiments, with each experiment performed in 4 replicate wells. (B) Soft agar co-culture of R2-T1AS cells and HuMEC cells. R2-T1AS cells were plated alone at a density of 1 × 104 cells per well (denoted as 1, red bar). In the R2-T1AS/HuMEC co-culture 1 × 104 R2-T1AS cells were mixed with 8 × 104 HuMEC cells (denoted as 8, brown stippled bar), and the R2-T1AS/G2B-10A co-culture was plated as in Figure 2A (red stippled bar). Graph shows mean colony numbers (± standard error) obtained in 5 independent experiments, with each experiment performed in 4 replicate wells. Different numbers for R2-T1AS-only cultures (65 in Figure 2A, and 5 in Figure 2B) reflect the experimental variability, as detailed in the text. * p < 0.05, ** p < 0.001, ns - not significant.