Figure 6
Effect of hypoxia on migration of ES and OS cell lines. (a) Fraction closure of a scratch wound due to cell migration during 18 h normoxia (white bars) or hypoxia (grey bars). (b) Effect of siRNA targeting HIF-1α (H1) or HIF-2α (H2) on migration of ES (combined data from A673, SK-ES-1 and SK-N-MC) or OS (combined data from MG63, Saos2 and 143B) cell lines under hypoxia. N, normoxia; H, hypoxia. Bar graph data is normalised to the (a) normoxic or (b) scrambled hypoxic siRNA control: *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, not significant.