Figure 2
HIF induction in ES and OS cell lines. Western blots showing induction of HIF-1α (120 kDa), HIF-2α (120 kDa) and Glut-1 (50 kDa) in a panel of ES (A673, RD-ES, SK-ES-1, SK-N-MC, TC-71) and OS (MG-63, Saos2, G292, 143B) cell lines in response to 24 h (a) hypoxia (H; 0.1% O2) or (b) low glucose (G; over-exposed in comparison to hypoxia blots) compared with the untreated/normoxic (N) control. (c) Activation of the HRE-luciferase reporter construct and (d) secretion of VEGF in response to hypoxia (light grey bars) or low glucose (dark grey bars). *, p < 0.05; **, p < 0.01; ***, p < 0.001.