Figure 3

PTEN is a target gene of miR-221 and miR-222. pGL3-PTEN construct containing PTEN 3'UTR was transfected into SGC7901 cells previously transfected with AS-miR-221 and/or AS-miR-222. Luciferase activity was determined 48 h after transfection. The ratio of normalized sensor to control luciferase activity is shown. Error bars represent the standard deviation and were obtained from three independent experiments (Figure 3A). Western blot analysis demonstrating that PTEN expression was significantly enhanced in AS-miR-221/222 treated cells, and significantly downregulated in the pMSCV-miR-221/222 infected group compared to controls (Figure 3B).