Figure 2

miR-221 and miR-222 affect phenotype of SGC7901 cells. MTT assay showing that cells transfected with AS-miR-221/222 proliferated at a significantly lower rate than controls (p = 0.0023). However, the cells infected with pMSCV-miR-221/222 proliferated at a significantly higher rate than controls (p = 0.0311) (Figure 2A). Flow cytometry analysis showing that the proportion of cells in the G1 phase in the AS-miR-221/222 group was significantly higher than that in the control group (p = 0.0036) and that the proportion of cells in the G1 phase in the pMSCV-miR-221/222 group was significantly lower than in the control group (p = 0.0266) (Figure 2B). Annexin V analysis showing that the cells transfected with AS-miR-221/222 underwent apoptosis at a significantly higher rate than controls(p = 0.0012), while cells infected with pMSCV-miR-221/222 underwent apoptosis at a significantly lower rate than controls (p = 0.0198) (Figure 2C). Transwell assay showing a decrease in invasive ability in the AS-miR-221/222 group and an increase in the pMSCV-miR-221/222 group compared to controls (Figure 2D). Clonogenic assay indicating that the radiosensitivity of SGC7901 cells increased in the AS-miR-221/222 group (p = 0.0032) and decreased in the pMSCV-miR-221/222 group compared with controls (p = 0.043) (Figure 2E).