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Table 2 Genomic methylation of cells treated with 5-aza-dC alone and in combination with TSA.

From: Demethylation by 5-aza-2'-deoxycytidine in colorectal cancer cells targets genomic DNA whilst promoter CpG island methylation persists

Time after 5-aza-dC

5-aza-dC & TSA

5-aza-dC alone

p-value

day 0

1.52%

1.18%

0.06

day 2

1.55%

1.52%

0.82

day 4

2.29%

2.32%

0.60

day 6

2.29%

2.37%

0.14

day 8

2.31%

2.38%

0.31

day 10

2.41%

2.39%

0.76

  1. Global methylation levels decreased with 5-aza-dC treatment and were gradually restored. T-test p-values indicate there is no synergistic effect caused by histone acetylation in the demethylation of genomic DNA.