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Table 1 Cell Death induced by 5-aza-dC and during recovery period.

From: Demethylation by 5-aza-2'-deoxycytidine in colorectal cancer cells targets genomic DNA whilst promoter CpG island methylation persists

Cell Line

Time Point

Viable (%)

Necrotic (%)

Apoptotic (%)

HCT116

Untreated

94.6

5.2

0.2

 

5-aza-dC treated

94.0

5.5

0.5

 

4 d post treatment

89.8

9.9

0.3

 

10 d post treatment

94.0

5.6

0.4

SW480

Untreated

99.8

0.1

0.1

 

5-aza-dC treated

80.3

14.2

5.5

 

4 d post treatment

90.5

8.7

1.4

 

10 d post treatment

92.6

6.2

1.2

LoVo

Untreated

78.4

9.4

12.2

 

5-aza-dC treated

67.2

7.0

25.8

 

4 d post treatment

67.0

10.7

22.3

 

10 d post treatment

77.6

11.3

11.1

  1. Necrosis and cells undergoing apoptosis increased after 5-aza-dC exposure. The number of necrotic cells remained elevated ten days post treatment in SW480 and LoVo whilst apoptosis was decreasing in all cell lines ten days post treatment.