Representative immunohistochemical staining for SNCG protein in human colon adenocarcinoma tissues (A-D) and Kaplan-Meier estimation of disease-free survival (E) and overall survival (F). A, SNCG expressed in the cytoplasm of colon cancer cells but not in the adjacent normal adenoepithelium. SNCG also expressed in colon neuron-chords, vascular endothelial cells, and smooth muscle cells of nearly all colon cancer specimens; B, C, and D represented the intensity of weak (1 score), moderate (2 scores) and strong (3 scores) staining of SNCG in cancer cytoplasm (original magnification 200×); E-F, Cases with SNCG-negative tumors (thick line) versus cases with SNCG-positive (thin line). SNCG-positive patients showed significantly poorer survival rates than those of SNCG-negative patients (P < 0.0001 by Log Rank (Mantel Cox) test). Numbers in the graph indicate percentages of DFS and OS at 5 year, respectively.